diacylglycerol kinase theta; DAG kinase theta; diglyceride kinase theta; DGK-theta (DGKQ, DAGK4)

Function

• phosphorylates diacylglycerol (DAG) to generate phosphatidic acid
• may regulate the activity of protein kinase C by controlling the balance between these two signaling lipids
• activated in the nucleus in response to alpha-thrombin & nerve growth factor (putative)
• may be involved in cAMP-induced activation of NR5A1 & subsequent steroidogenic genetranscription by delivering phosphatidic acid as ligand for NR5A1
• acts synergistically with NR5A1 on CYP17 transcriptional activity
• inactivated by binding to RHOA
• not inhibited by phosphatidylserine
• phosphorylated by PRKCE & PRKCH in vitro
• interacts with RHOA (constitutively activated, GTP-bound); the interaction inhibits DGKQ. interacts with PRKCE
• interacts with PRKCH
• interacts with PLCB1
• interacts with NR5A1; interaction requires the LXXLL motif 1 & LXXLL motif 2 in DGKQ

ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate

Structure

• the L-X-X-L-L repeats are implicated in binding to the nuclear receptor NR5A1
• belongs to the eukaryotic diacylglycerol kinase family
• contains 1 DAGKc domain
• contains 3 phorbol-ester/DAG-type Zn+2 fingers
• contains 1 Ras-associating domain

Compartment

• cytoplasm, cell membrane, cytoskeleton
• nucleus, nuclear speckle
• translocates to the nucleus in response to thrombin stimulation (putative)
• translocates to the plasma membrane in response to steroid hormone receptor stimulation
• translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation & subsequent activation of PRKCE & probably PRKCH

More general terms

• diacylglycerol kinase
• zinc finger protein
• phosphoprotein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=1609
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:1609
• OMIM: https://mirror.omim.org/entry/601207
• UniProt: http://www.uniprot.org/uniprot/P52824.html