Rho guanine nucleotide exchange factor 1; 115 kD guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; sub1.5 (ARHGEF1)

Function

• seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) & guanine nucleotide-binding alpha-13 (GNA13) subunits
• acts as GTPase-activating protein (GAP) for GNA12 & GNA13, & as guanine nucleotide exchange factor (GEF) for RhoA GTPase
• activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain
• RhoGEF activity is inhibited by binding to activated GNA12
• mediates angiotensin-2-induced RhoA activation
• phosphorylated by PKCA
• angiotensin-2 induced Tyr-738
• phosphorylation is mediated by JAK2
• interacts with RHOA, GNA12 & GNA13
• homooligomerizes through the coiled coil region
• may interact with CCPG1
• interacts with CTNNAL1

Structure

• the RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity
• the DH domain is involved in interaction with CCPG1
• contains 1 DH (DBL-homology) domain
• contains 1 PH domain
• contains 1 RGSL (RGS-like) domain

Compartment

• cytoplasm, membrane
• translocated to the membrane by activated GNA13 or LPA stimulation

Alternative splicing

named isoforms=3

Expression

ubiquitously expressed

More general terms

• rho guanine nucleotide exchange factor
• phosphoprotein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=9138
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:9138
• OMIM: https://mirror.omim.org/entry/601855
• UniProt: http://www.uniprot.org/uniprot/Q92888.html