crossover junction endonuclease MUS81; Holliday junction resolvase Mus81 (MUS81)

Function

• interacts with EME1 & EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick
• typical substrates include 3'-flap structures, replication forks & nicked Holliday junctions
• may be required in mitosis for the processing of stalled or collapsed replication forks
• may self-associate
• interacts with EME1, EME2 & CHEK2
• interacts with BLM
  • interaction may stimulate endonuclease activity of MUS81
• interacts with SLX4/BTBD12
  • interaction links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair
• interacts with DCLRE1B/apollo

Cofactor: Mg+2

Structure

• belongs to the XPF family
• contains 1 ERCC4 domain

Compartment

• nucleus, nucleolus.
• recruited to foci of DNA damage in S-phase cells

Expression

• widely expressed
• expressed in S phase & G2 phase
• induced by in cells treated with agents that damage DNA or block replication, independent of transcription (putative)

More general terms

• endodeoxyribonuclease
• nuclear protein
• phosphoprotein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=80198
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:80198
• OMIM: https://mirror.omim.org/entry/606591
• UniProt: http://www.uniprot.org/uniprot/Q96NY9.html