Crossover junction endonuclease EME1; MMS4 homolog; hMMS4 (EME1, MMS4)

Function

• interacts with MUS81 to form a DNA structure-specific endonuclease - substrate preference for branched DNA with 5'-end at the branch nick
• substrates include
  • 3'-flap structures
  • replication forks
  • nicked Holliday junctions.
• processing of stalled or collapsed replication forks
• interacts with MUS81, ERCC4 & FANCM

Cofactor: Mg+2

Compartment

• nucleus, nucleolus
• recruited to regions of DNA damage in S-phase cells

Alternative splicing

named isoforms=2

More general terms

• endodeoxyribonuclease
• phosphoprotein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=146956
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:146956
• OMIM: https://mirror.omim.org/entry/610885
• UniProt: http://www.uniprot.org/uniprot/Q96AY2.html