protein prune homolog (hPrune, drosophila-related expressed sequence 17, DRES-17, DRES17, HTcD37, PRUNE)

Function

• phosphodiesterase (PDE)
• higher activity toward cAMP than cGMP, as substrate
• plays a role in cell proliferation
• able to induce cell motility
• acts as a negative regulator of NME1
• interacts with NME1
• interacts with GSK3; at focal adhesion complexes where paxillin & vinculin are colocalized

diphosphate + H2O <-->  2 phosphate

Cofactor:

• Mg+2 activates

Inhibition:

• Mn+2
• dipyridamole
• moderately sensitive to IBMX
• vinpocetine

Kinetic parameters:

• KM=0.91 uM for cAMP
• KM=2.3 uM for cGMP
• Vmax=12.8 pmol/min/ug enzyme with cAMP as substrate
• Vmax=0.8 pmol/min/ug enzyme with cGMP as substrate

Structure

• homooligomer
• able to homodimerize via its C-terminal domain
• belongs to the PPase class C family, Prune subfamily

Compartment

• cytoplasm, nucleus
• cell junction, focal adhesion.
• in some transfected cells a nuclear staining is also observed

Alternative splicing

named isoforms=6

Expression

• ubiquitously expressed

Pathology

• seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas & malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas

More general terms

• phosphodiesterase

Database

• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:5849
• UniProt: http://www.uniprot.org/uniprot/Q86TP1.html