death-associated protein kinase 3; DAP kinase 3; DAP-like kinase; Dlk; ZIP-kinase (DAPK3, ZIPK)

Function

• serine/threonine kinase
• acts as a positive regulator of apoptosis
• phosphorylates histone H3 on Thr-11 at centromeres during mitosis
• regulates myosin light chain phosphatase via phosphorylation of MYPT1 thus regulating assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction & neurite outgrowth
• phosphorylated on Thr-265 & Thr-299 by ROCK1 which activates the kinase
• autophosphorylated
• homodimer or forms heterodimers with ATF4; both interactions require an intact leucine zipper domain
• oligomerization is required for full enzymatic activity
• also binds to DAXX & PAWR, possibly in a ternary complex which plays a role in caspase activation
• interacts with AATF & CDC5L

Cofactor: Mg+2

Structure

• belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, DAP kinase subfamily
• contains 1 protein kinase domain

Compartment

• nucleus, cytoplasm (putative)
• relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (putative)
• associates to centromeres from prophase to anaphase

More general terms

• serine/threonine kinase
• pro apoptotic protein
• nuclear protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=1613
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:1613
• OMIM: https://mirror.omim.org/entry/603289
• UniProt: http://www.uniprot.org/uniprot/O43293.html