CDK7/cyclin H complex, CDK-activating kinase (CAK) or p34cdc2 activating kinase
Jump to navigation
Jump to search
Function
- CAK is involved in controlling multiple cell cycle transitions phosphorylating many CDK/cyclin complexes
- activation of CAK occurs through phosphorylation of Ser170 & Thr176 (presumably on CDK7) by another serine/threonine kinase
- CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 & CDK6 by threonine phosphorylation
- CAK is a component of TFIIH & in this form phosphorylates the CTD of RNA polymerase 2 L' subunit[3]; this allows escape from the promoter & elongation of the transcripts
- most CAK though is found separate from TFIIH
- cyclin H/cdk7 complex preferentially phosphorylates cdk2 over RNA polymerase 2 CTD, however CAK & TFIIH show the opposite preference
- TFIIH (not CAK) can also phosphorylate TFIIE & TFIIF[4]
- kinase activity of CAK towards CDK's appears to be regulated largely by cyclin binding
- CAK phosphorylates p34cdc2 on Thr161
Structure
More general terms
References
- ↑ Solomon MJ. The function(s) of CAK, the p34cdc2-activating kinase. Trends Biochem Sci. 1994 Nov;19(11):496-500. PMID: https://www.ncbi.nlm.nih.gov/pubmed/7855894
- ↑ 2.0 2.1 Morgan DO. Principles of CDK regulation. Nature. 1995 Mar 9;374(6518):131-4. Review. PMID: https://www.ncbi.nlm.nih.gov/pubmed/7877684 (review CDK regulation)
- ↑ 3.0 3.1 Shiekhattar R, Mermelstein F, Fisher RP, Drapkin R, Dynlacht B, Wessling HC, Morgan DO, Reinberg D. Cdk-activating kinase complex is a component of human transcription factor TFIIH. Nature. 1995 Mar 16;374(6519):283-7. PMID: https://www.ncbi.nlm.nih.gov/pubmed/7533895 (presence in TFIIH)
- ↑ 4.0 4.1 Dynlacht BD. Regulation of transcription by proteins that control the cell cycle. Nature. 1997 Sep 11;389(6647):149-52. Review. PMID: https://www.ncbi.nlm.nih.gov/pubmed/9296491
- ↑ UniProt http://www.uniprot.org/uniprot/P51946.html