protein-methionine sulfoxide oxidase MICAL1; molecule interacting with CasL protein 1; MICAL-1; NEDD9-interacting protein with calponin homology & LIM domains (MICAL1, MICAL, NICAL)

Function

• monooxygenase
• promotes depolymerization of F-actin by mediating oxidation of specific Met on actin
• acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to actin filament severing & inhibition of repolymerization (probable)
• acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments
• also acts as a negative regulator of apoptosis via its interaction with STK38 & STK38L
• acts by antagonizing STK38 & STK38L activation by MST1/STK4
• interacts with STK38 & STK38L (putative)
• associates with the SH3 domain of NEDD9
• interacts with VIM & PLXNA3
• interacts with RAB1B

[protein]-methionine + NADPH + O2 =

[protein]-methionine-sulfoxide + NADP+ + H2O

Cofactor: FAD

Kinetic parameters:

• KM=4.7 uM for F-actin (at saturing NADPH concentration)

Structure

• the C-terminal coiled coil part contains the plexin- interacting region
• belongs to the mical family
• contains 1 CH (calponin-homology) domain
• contains 1 LIM Zn+2-binding domain

Cofactor: FAD (putative)

Compartment

cytoplasm, cytoskeleton

Alternative splicing

named isoforms=4

Expression

• expressed in the thymus, lung, spleen, kidney, testis, hematopoietic cells

More general terms

• hydroxylase; monooxygenase
• cytoskeletal protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=64780
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:64780
• OMIM: https://mirror.omim.org/entry/607129
• UniProt: http://www.uniprot.org/uniprot/Q8TDZ2.html