replication protein A 32 kD subunit; RP-A p32; RP-A p34; replication factor A protein 2; RF-A protein 2 (RPA2 REPA2 RPA32 RPA34)
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Function
- subunit of replication factor A
- RPA2 is phosphorylated when cells traverse the G1->S boundary; phosphorylated through mitosis
- RPA2 becomes hyperphosphorylated after ionizing radiation or UV radiation
- phosphorylation of RPA2 by p34cdc2 (CDK1) may activate RPA activity & DNA replication; hyperphosphorylation may inactivate RPA, halt DNA replication & enhance DNA repair
- phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci
- can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku & DNA, & by CDC2
- binds to SERTAD3/RBT1
- interacts with TIPIN
Compartment
- nucleus
- also present in PML nuclear bodies
- redistributes to discrete nuclear foci upon DNA damage
Alternative splicing
named isoforms=3
More general terms
Component of
References
- ↑ UniProt http://www.uniprot.org/uniprot/P15927.html
- ↑ NIEHS-SNPs http://egp.gs.washington.edu/data/rpa2/
- ↑ Linn S How many pols does it take to replicate nuclear DNA? Cell 66:185 1991 PMID: https://www.ncbi.nlm.nih.gov/pubmed/1855251
- ↑ Gottlieb TM, Jackson SP. Protein kinases and DNA damage. Trends Biochem Sci. 1994 Nov;19(11):500-3. Review. PMID: https://www.ncbi.nlm.nih.gov/pubmed/7855895
- ↑ de Laat WL et al, Molecular mechonisms of nucleotide excision repair. Genes & Dev 13:768-85, 1999 PMID: https://www.ncbi.nlm.nih.gov/pubmed/10197977