double stranded DNA-dependent protein kinase (DNA-PK)

^[1]^[2]^[3]

Function

may function as a primary DNA-damage sensor
in vitro, DNA-PK requires ds DNA ends or other discontinuities in the DNA double helix
once bound to DNA, DNA-PK can translocate into the body of DNA to access distal targets
may also be involved in V(D)J recombination as well as DNA double-strand break repair

trimolecular complex consisting of a 350 kD catalytic subunit & a DNA-targeting component, the human autoimmune antigen Ku

SCID mice show deficiencies in p350DNA-PK^[2] & a radio-sensitive cell line was found to be deficient in p350DNA-PK^[3]

↑ Gottlieb TM, Jackson SP. Protein kinases and DNA damage. Trends Biochem Sci. 1994 Nov;19(11):500-3. Review. PMID: https://www.ncbi.nlm.nih.gov/pubmed/7855895
↑ ^2.0 ^2.1 Kirchgessner CU, Patil CK, Evans JW, Cuomo CA, Fried LM, Carter T, Oettinger MA, Brown JM. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science. 1995 Feb 24;267(5201):1178-83. PMID: https://www.ncbi.nlm.nih.gov/pubmed/7855601
↑ ^3.0 ^3.1 Lees-Miller SP, Godbout R, Chan DW, Weinfeld M, Day RS 3rd, Barron GM, Allalunis-Turner J. Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line. Science. 1995 Feb 24;267(5201):1183-5. PMID: https://www.ncbi.nlm.nih.gov/pubmed/7855602