RuvB-like 1 (49-kD TATA box-binding protein-interacting protein, 49 kD TBP-interacting protein, TIP49a, Pontin 52, nuclear matrix protein 238, NMP 238, 54 kD erythrocyte cytosolic protein, ECP-54, TIP60-associated protein 54-alpha, TAP54-alpha, RUVBL1, NMP238, TIP49, TIP49A)

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Function

possesses single-stranded DNA-stimulated ATPase & ATP-dependent DNA helicase (3' to 5') activity
component of the NuA4 histone acetyltransferase complex
NuA4 complex ATPase & helicase activities contributed by association of RUVBL1 & RUVBL2 with EP400
RUVBL1 plays an essential role in oncogenic transformation by MYC & also modulates transcriptional activation by the LEF1/TCF1- CTNNB1 complex
may be able to bind plasminogen at cell surface & enhance plasminogen activation
essential for cell proliferation
forms homotypic & heterotypic interactions
forms a multimeric complex with RUVBL2
interacts with the transcriptional activation domain of MYC
component of the RNA polymerase 2 holoenzyme complex
may also act to bridge the LEF1/TCF1-CTNNB1 complex & TBP
associates with alpha & gamma tubulins, particularly during metaphase & early anaphase
interacts with NPAT

nuclear matrix, nucleus, nucleoplasm cytoplasm, membrane
mainly localized in the nucleus
associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm & associated with the cell membranes
in prophase & prometaphase, it is located at the centrosome & the branching microtubule spindles
after mitotic nuclear membrane disintigration it accumulates at the centrosome & sites of tubulin polymerization
as cells pass through metaphase & into telophase it is located close to the centrosome at the early phase of tubulin polymerization
in anaphase, it accumulates at the zone of tubule interdigitation
in telophase, it is found at polar tubule overlap, & it reappears at the site of chromosomal decondensation in the daughter cells

high level of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases including polymyositis/dermatomyosistis & autoimmune hepatitis