carbohydrate sulfotransferase 4; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GST-3; high endothelial cells N-acetylglucosamine 6-O-sulfotransferase; HEC-GlcNAc6ST; L-selectin ligand sulfotransferase; LSST; N-acetylglucosamine 6-O-sulfotransferase 2; GlcNAc6ST-2; Gn6st-2 (CHST4)

Function

• catalyzes transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as L-selectin ligands
• L-selectin ligands are present in high endothelial cells & play a central role in lymphocyte homing at sites of inflammation
• participates in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X on L-selectin counter receptors CD43, GlyCAM-1 & MAdCAM-1
• also involved in biosynthesis of L-selectin ligand recognized by MECA-79 antibody
• plays a central role in lymphocyte trafficking during chronic inflammation
• catalytic preference for core 2-branched mucin-type O-glycans
• can use GlcNAcbeta1-6[galbeta1-3]galNAc-pNP (core 2), GlcNAcbeta1-6manOMe & GlcNAcbeta1-2man oligosaccharide structures as acceptors
• has activity toward core 3 of GlcNAcbeta1-3galNAc-pNP
• substrate specificity may be influenced by its subcellular location

Structure

• monomer
• belongs to the sulfotransferase 1 family gal/GlcNAc/galNAc subfamily

Compartment

• Golgi membrane
• single-pass type 2 membrane protein

Expression

• specifically expressed in high endothelial venules
• weakly expressed in spleen
• not expressed in other tissues
• upon cytokine activation, it is expressed at low level

Pathology

• expressed in colonic mucinous adenocarcinoma

Pharmacology

• may serve as a antiinflammatory target

More general terms

• carbohydrate sulfotransferase (CHST)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=10164
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:10164
• UniProt: http://www.uniprot.org/uniprot/Q8NCG5.html