E3 ubiquitin-protein ligase MYLIP; inducible degrader of the LDL-receptor; Idol; myosin regulatory light chain interacting protein; MIR (MYLIP, BZF1, IDOL, BM-023, PP5242)

Function

• E3 ubiquitin-protein ligase
• mediates ubiquitination & subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR & LRP8
• proteasomal degradation of MRLC inhibits neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) & reducing CNPY2-stimulated neurite outgrowth
• acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination & subsequent degradation of LDLR
• protein modification; protein ubiquitination
• autoubiquitinated
• interacts with myosin regulatory light chain (MRLC) & TMEM4

Structure

• the RING domain mediates ubiquitination & the neurite outgrowth inhibitory activity
• contains 1 FERM domain
• contains 1 RING-type Zn+2 finger

Compartment

cytoplasm (probable)

Alternative splicing

named isoforms=2

Expression

• ubiquitously expressed
• expressed in fetal tissues
• higher expression in placenta & fetal lung
• induced expression is directly activated by NR1H2 & NR1H3
• expression is indirectly induced by LDL
• not sterol-response element-binding protein (SREBP) dependent

More general terms

• E3 ubiquitin ligase
• ring finger protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=29116
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:29116
• OMIM: https://mirror.omim.org/entry/610082
• UniProt: http://www.uniprot.org/uniprot/Q8WY64.html