DNA damage up-regulated protein; DDUP (CTBP1-DT)
Jump to navigation
Jump to search
Function
- promotes DNA damage repair through both homologous recombination repair (HRR) & post-replication repair (PRR) mechanisms
- enhances retention of DNA damage response protein RAD18 at sites of DNA damage
- this allows for HRR via association of RAD18 with RAD51C & for PRR via RAD18-mediated promotion of PCNA monoubiquitination
- phosphorylated in an ATR-dependent manner
- phosphorylation is required for interaction with H2AX & RAD18 & for DDUP-mediated DNA damage repair
- interacts with DNA damage response proteins ATR, H2AX, PCNA, RAD18 & RAD51C
- forms a complex with H2AX & RAD18 following DDUP phosphorylation
Compartment
- nucleus, chromosome
- forms DNA damage-induced foci
Expression
induced by DNA damage
Pharmacology
- treatment of cells with ATR inhibitor Berzosertib abolishes the interaction of DDUP with H2AX, RAD18 & RAD51C & inhibits formation of DDUP foci, leading to hypersensitivity of cancer cells to DNA-damaging chemotherapeutics