CREST-BRG1 complex

Function

• regulates promoter activation by orchestrating Ca+2-dependent release of a repressor complex & a recruitment of an activator complex
• in resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex
• upon Ca+2 influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex
• at the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation
• the CREST-BRG1 complex also binds to the NR2B promoter, & activity-dependent induction of NR2B expression involves a release of HDAC1 & recruitment of CREBBP

Structure

• subunits: SMARCA4/BRG1/BAF190A, SS18L1/CREST, HDAC1, RB1 & SP1

More general terms

• molecular complex