calpain-8; stomach-specific M-type calpain; new calpain 2; nCL-2 (CAPN8, NCL2)

Function

• Ca+2-regulated non-lysosomal thiol-protease
• involved in membrane trafficking in gastric surface mucus cells (pit cells) & may involve the membrane trafficking of mucus cells via interactions with coat protein
• proteolytically cleaves the beta-subunit of coatomer complex (putative)
• undergoes autolytic cleavage between Ala-5 & Ala-6 which gives rise to fragments extending from Ala-6 to the C-terminus, Ala-6 to the EF-hand 2 domain & from Ala-6 to the beginning of domain III (putative)
• broad endopeptidase specificity
• interacts with GPS1, COPB1, EYA2, NME2, NME4 & TOMM70A (putative)

Cofactor: binds 2 Ca+2 (putative)

Structure

• monomer & homoligomer
• domain III mediates oligomerization (putative)
• belongs to the peptidase C2 family
• contains 1 calpain catalytic domain
• contains 3 EF-hand domains

Compartment

• cytoplasm (putative)
• Golgi

Expression

stomach

More general terms

• calpain

Database

• UniProt: http://www.uniprot.org/uniprot/A6NHC0.html