amylase in serum/plasma
Indications
- evaluation of acute pancreatitis
Reference interval
- Male & Female: Serum: 30- 110 U/L
Principle
Increases
- acute pancreatitis
- serum levels rise within 6-48 hours after onset of acute pancreatitis in about 80% of patients
- values in excess of 4 times the upper limit of normal are highly suggestive of pancreatitis
- activity of amylase in serum generally returns to normal within 3-5 days in patients with milder forms of acute pancreatitis
- elevated serum amylase persisting more than 3-5 days suggests pancreatic pseudocyst or pancreatic necrosis[4]
- use in conjunction with serum lipase improves specificity
- mumps, parotitis
- intestinal obstruction
- intestinal ischemia, intestinal infarction, appendicitis
- renal failure
- also see hyperamylasemia
- pharmaceutical agents:
- in vivo effects
Decreases
Specimen
No special patient preparation is necessary.
Sample preparation: Collect the specimen by standard venipuncture technique. Use Lithium or sodium heparin as an anticoagulant for plasma specimens. Plasma values are approximately 20 uL higher than serum values.
Urine: Collect timed or random specimens by standard laboratory procedures. Timed specimens are preferred: DO NOT USE URINE CONTAINING CHELATING AGENTS. (e.g. citrates, oxalates, or EDTA)
Special Precautions:
Serum or Plasma: See Sample Dilution for grossly lipemic specimens. Refrigerate or freeze samples that cannot be analyzed immediately.
Do not use calcium-chelating anticoagulants such as oxalates, citrated, & EDTA.
Minimum sample size 0.5 milliliters with an optimum size of 1.0 milliliter or larger.
More general terms
More specific terms
Additional terms
Component of
- amylase + triacylglycerol lipase in serum/plasma
- pancreatic panel
- macroamylase/amylase in serum/plasma
References
- ↑ Kodak Ektachem 700 Analyzer Operator's Manual, Kodak Clinical Products, Rochester, New York.
- ↑ Kodak Ektachem Slide Package Inserts, Kodak Clinical Products Rochester, New York.
- ↑ Kodak Ektachem Training Manual, Kodak Clinical Products, Rochester, New York.
- ↑ 4.0 4.1 Henry's Clinical Diagnosis & Management by Laboratory Methods, 21st edition, McPherson RA & Pincus MR (es), W.B. Saunders Co., Philadelphia, PA. 2007, pg 287
- ↑ Amylase, Plasma or Serum Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0020013.jsp
- ↑ Panel of 3 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0020804.jsp
- ↑ Panel of 3 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0000000.jsp