katanin p60 ATPase-containing subunit A1; katanin p60 subunit A1; p60 katanin (KATNA1)
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Function
- severs microtubules in vitro (ATP-dependent)
- rapid reorganization of cellular microtubule arrays (putative)
- disassembly of interphase microtubules at G2-M transition
- microtubule release from the centrosome after nucleation
- depolymerization of microtubule end of mitotic spindles, proximal to centrosome, & subsequent poleward microtubule flux
- in neurons, microtubule release within cell body may allow their subsequent transport into axons by microtubule dependent motor proteins; this transport is required for axonal growth
- ATPase activity is stimulated by microtubules, which promote homooligomerization
- ATP dependent microtubule severing is stimulated by interaction KATNB1
- homooligomerizes into hexameric rings, promoted by microtubules
- interacts with KATNB1, targeting subunit (putative)
- phosphorylation by DYRK2 triggers ubiquitination & subsequent degradation
- ubiquitinated by the EDVP E3 ligase complex & subsequently targeted to proteasomal degradation
- associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 & VPRBP proteins (EDVP complex)
Structure
- the N-terminus is sufficient for interaction with microtubules, although high affinity binding requires an intact C-terminal domain & ATP, which promotes oligomerization
- belongs to the AAA ATPase family
Compartment
- cytoplasm, cytoskeleton, centrosome, spindle pole
- predominantly cytoplasmic
- also localized to the interphase centrosome
- recruitment to the mitotic spindle poles requires microtubules & interaction with KATNB1
Alternative splicing
named isoforms=2