multivesicular body subunit 12A; CIN85/CD2AP family-binding protein; ESCRT-I complex subunit MVB12A; protein FAM125A (MVB12A, CFBP, FAM125A)

Function

• component of the ESCRT-1 complex, a regulator of vesicular trafficking process
• required for sorting of endocytic ubiquitinated cargos into multivesicular bodies
• may be involved in the ligand-mediated internalization & down-regulation of EGF receptor
• phosphorylated on Tyr-204 upon EGF stimulation
• phosphorylation is required for interaction with CD2AP & CIN85/SH3KBP1
• interacts with CD2AP & CIN85/SH3KBP1
• interacts with CD2AP (via one of the SH3 domains)
• within the ESCRT-1 complex
  • interacts with VPS28
  • interacts with TSG101, VPS37B, VPS37C, & VPS37D
    • association appears to be mediated by the TSG101-VPS37 binary subcomplex
• interacts with CEP55
• does not interact with CD2AP

Structure

• belongs to the MVB12 family
• contains 1 MABP domain
• contains 1 UMA domain

Compartment

• cytoplasm, nucleus
• endosome, late endosome membrane
• cytoskeleton, microtubule organizing center, centrosome
• colocalizes with F-actin
• some fraction may be nuclear

Alternative splicing

named isoforms=3

Expression

• ubiquitously expressed
• not expressed in skeletal muscle

More general terms

• cytoskeletal protein
• phosphoprotein

Component of

• ESCRT-1 complex; endosomal sorting complex required for transport 1

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=93343
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:93343
• UniProt: http://www.uniprot.org/uniprot/Q96EY5.html