kelch domain-containing protein 5; kelch-like protein 42; Cullin-3-binding protein 9; Ctb9 (KLHL42, KIAA1340, KLHDC5)

Function

• component of the BCR(KLHL42) E3 ubiquitin ligase complex
  • substrate-specific adapter of the complex
  • required for progression & cytokinesis
  • the BCR(KLHL42) E3 ubiquitin ligase complex mediates the ubiquitination & subsequent degradation of KATNA1
  • role in microtubule dynamics throughout mitosis
  • interacts (via the BTB domain) with CUL3.
  • interacts (via the kelch domains) with KATNA1
• phosphorylated upon DNA damage, probably by ATM or ATR

Structure

• contains 1 BTB (POZ) domain
• contains 6 Kelch repeats

Compartment

• cytoplasm, cytoskeleton, spindle
• predominantly in mitotic cells
• localized diffusely in the cytoplasm during the interphase
• during metaphase is localized throughout the cell & more widely dispersed than the microtubules
• in anaphase cells is localized between the two sets of separated chromosomes as well as at the spindle poles
• during telophase is localized arround the nuclei of the two daughter cells
• not detected at the midbody region during cytokinesis

Expression

up-regulated during mitosis

More general terms

• Kelch repeat & BTB domain containing protein
• kelch-like protein
• phosphoprotein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=57542
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:57542
• UniProt: http://www.uniprot.org/uniprot/Q9P2K6.html