chromatin assembly factor 1 subunit A; CAF-1 subunit A; chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-Ip150 (CHAF1A, CAF, CAF1P150)

Function

• component of nuclear complexes:
  • CCR4-NOT core complex
  • chromatin assembly factor 1 (CAF-1 complex)
    • CHAF1A binds directly to CHAF1B within the complex
    • CHAF1A binds to histones H3 & H4
    • may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci
• CHAF1A binds PCNA, CBX1, MBD1, CBX5
• during DNA replication, CHAF1A forms a S phase-specific complex that facilitates DNA methylation & histone H3 'Lys-9' methylation during replication-coupled chromatin assembly
  • the complex is at least composed of CHAF1A, MBD1 & SETDB1

Structure

• homodimer
• contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif required for interaction with chromoshadow domains
  • this motif requires additional residues -7, -6, +4 & +5 of the central Val which contact the chromoshadow domain
• belongs to the CHAF1A family

Compartment

nucleus, DNA replication fork

Alternative splicing

named isoforms=3

Expression

• active complex is found in G1, S & G2 phases

More general terms

• protein subunit
• phosphoprotein

Component of

• CCR4-NOT core complex
• chromatin assembly factor 1 (CAF-1)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=10036
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:10036
• OMIM: https://mirror.omim.org/entry/601246
• UniProt: http://www.uniprot.org/uniprot/Q13111.html