centrosomal protein 2; centrosome-associated protein CEP250; 250 kD centrosomal protein; Cep250; centrosomal Nek2-associated protein 1; C-Nap1 (CEP250, CEP2, CNAP1)

Function

• probably plays a role in centrosome cohesion during interphase
• differentially phosphorylated during cell cycle
• phosphorylation may regulate association/dissociation from centrosome
• during M phase of mitosis, C-terminal part is phosphorylated by NEK2, suggesting that it may trigger the dissociation from the mitotic centrosome
• dephosphorylated in vitro by the PP1 phosphatase
• interacts with CROCC/rootletin (putative)
• forms a complex in vitro with both NEK2 kinase & the PPP1CC catalytic subunit of protein phosphatase 1 (PP1)
• interacts with CEP135

Structure

• monomer & homodimer (probable)

Compartment

• cytoplasm, perinuclear region
• cytoskeleton, centrosome, centriole, cilium basal body
• component of the core centrosome
• in interphase cells, it specifically associates with the proximal ends of both mother & daughter centrioles
• associates with the centrosome in interphase cells
• in mitotic cells, it dissociates from the mitotic spindle poles
• at the end of cell division, it reaccumulates at centrosomes
• in photoreceptors, found at the proximal ends of basal bodies

Alternative splicing

named isoforms=2

Expression

ubiquitously & weakly expressed

Pathology

• antibodies against CEP2 are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins

More general terms

• centrosomal protein
• phosphoprotein

Component of

• condensin

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=11190
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:11190
• OMIM: https://mirror.omim.org/entry/609689
• UniProt: http://www.uniprot.org/uniprot/Q9BV73.html