carbohydrate sulfotransferase 8; galNAc-4-O-sulfotransferase 1; galNAc-4-ST1; galNAc4ST-1; N-acetylgalactosamine-4-O-sulfotransferase 1 (CHST8)

Function

• catalyzes transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (galNAc) residues in both N-glycans & O-glycans
• required for biosynthesis of glycoprotein hormones lutropin & thyrotropin, by mediating sulfation of their carbohydrate structures
• only active against terminal galNAcbeta1,galNAcbeta
• not active toward chondroitin

Kinetic parameters:

• KM=10 uM for carbonic anhydrase VI
• pH dependence: optimum pH is 7.2

Structure

belongs to the sulfotransferase 2 family

Compartment

• Golgi membrane
• single-pass type 2 membrane protein (putative)

Expression

• predominantly expressed in pituitary gland
• in brain, expressed in pituitary gland, cerebellum, medulla oblongata, pons, thalamus & spinal cord
• expressed at lower level in lung, spleen, adrenal gland, placenta, prostate, testis, mammary gland & trachea

Pathology

• down-regulation of CHST8 (17-fold) in prion-infected cells

More general terms

• carbohydrate sulfotransferase (CHST)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=64377
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:64377
• OMIM: https://mirror.omim.org/entry/610190
• UniProt: http://www.uniprot.org/uniprot/Q9H2A9.html