cerebrospinal fluid (CSF) protein electrophoresis
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Introduction
CSF protein electrophoresis (after concentration)
Characteristics
- CSF is an ultrafiltrate of plasma
- all proteins visible on electrophoresis are <150 kD
- prominent prealbumin band
- 2 transferrin bands
- beta-1 transferrin
- beta-2 transferrin (CSF-specific transferrin)
Indications
- used largely to detect intrathecal production of IgG
- IgG/albumin ratio < 0.27 considered normal
- multiple sclerosis
- beta-2 transferrin presence used for establishing CSF leakage
Reference interval
Table
| analyte | %* | value | plasma/CSF ratio |
|---|---|---|---|
| total protein | - | 5-24 mg/dL | - |
| - prealbumin | 2-7% | 17.3 mg/L | 14 |
| - albumin | 56-76% | 155 mg/L | 260 |
| - alpha-1 globulin | 2-7% | - | - |
| - alpha-2 globulin | 4-12% | - | - |
| - beta globulin | 8-18% | - | - |
| - gamma globulin | 3-12% | - | - |
* % of total protein <also see CSF analysis>
More general terms
More specific terms
- monoclonal protein in CSF
- oligoclonal banding in CSF (high-resolution CSF protein electrophoresis)
- oligoclonal bands in serum&CSF
Additional terms
References
- ↑ Fundamentals of Clinical Chemistry 3rd ed., N.W. Teitz ed., W.B. Saunders, 1988, pg 339
- ↑ Saunders Manual of Medical Practice, Rakel (ed), WB Saunders, Philadelphia, 1996, pg 1065-67
- ↑ Tietz Textbook of Clinical Chemistry, 2nd ed. Burtis CA & Ashwood ER (eds), WB Saunders Co, Philadelphia PA, 1993
- ↑ Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996