charged multivesicular body protein 4C; chromatin-modifying protein 4c; CHMP4c; SNF7 homolog associated with Alix 3; SNF7-3; hSnf7-3; vacuolar protein sorting-associated protein 32-3; Vps32-3; hVps32-3 (CHMP4C, SHAX3)

Function

• component of ESCRT3 in the multivesicular body pathway
• in the ESCRT3 complex, it interacts directly with CHMP4A, CHMP4B, CHMP6
• self-associates
• interacts with PDCD6IP of ESCRT1 complex
• interacts with CHMP2A
• interacts with VPS4A

Structure

• the acidic C-terminus & the basic N-termminus are thought to render the protein in a closed, soluble & inactive conformation through an autoinhibitory intramolecular interaction
• the open & active conformation, which enables membrane binding & oligomerization, is facilitated by interaction with other ESCRT components (putative)
• belongs to the SNF7 family

Compartment

cytoplasm, late endosome membrane

Expression

expressed in heart, spleen, kidney

Pathology

• the HIV-1 virus takes advantage of ESCRT3 for budding & exocytosis of viral proteins, via the association of CHMP4 proteins with PDCD6IP, a protein directly recruited by HIV-1 p6 protein that functions at sites of viral Gag assembly & budding
• overexpression strongly inhibits HIV-1 release

More general terms

• charged multivesicular body protein (CHMP)

Component of

• endosomal sorting complex required for transport-3 (ESCRT3)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=92421
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:92421
• OMIM: https://mirror.omim.org/entry/610899
• UniProt: http://www.uniprot.org/uniprot/Q96CF2.html