mitogen-activated protein kinase kinase kinase 13 (mixed lineage kinase, MLK, leucine zipper-bearing kinase, MAP3K13, LZK)

Function

• activates JUN N-terminal pathway through activation of MAP2K7
• acts synergistically with PRDX3 to regulate activation of NF-kappa-B in the cytosol
• activation is kinase-dependent & involves activating the IKK complex that phosphorylates inhibitors of NF-kappa-B
• activated by autophosphorylation & homodimerization
• autophosphorylated on Ser & Thr

ATP + a protein <--> ADP + a phosphoprotein

Cofactor: Mg+2 (putative)

Structure

• homodimer; forms dimers through the leucine-zipper motif
• interacts with the C-terminus of MAPK8IP1 through the kinase catalytic domain
• binds PRDX3
• associates with the IKK complex through the kinase domain
• belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
• contains 1 protein kinase domain

Compartment

cytoplasm, peripheral membrane

Alternative splicing

named isoforms=2

Expression

• expressed in the adult brain, liver, placenta & pancreas
• expression strongest in pancreas

More general terms

• MAP3K/MEKK

Database

• OMIM: https://mirror.omim.org/entry/604915
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=9175
• UniProt: http://www.uniprot.org/uniprot/O43283.html