apolipoprotein A1/B in serum
Indications
- assess risk of coronary atherosclerosis; ApoA1/ApoB100 ratio is better than LDL/HDL ratio[5]
- establish diagnosis of dyslipidemia
- provide additional information on the effectiveness of dietary & pharmacologic management of lipid disorders
Reference interval
Table
| analyte | gender | value | |
|---|---|---|---|
| ApoA1 | male | 96-176 mg/dL | |
| ApoA1 | female | 109-203 mg/dL | |
| ApoB100 | male | 43-128 mg/dL | |
| ApoB100 | female | 42-112 mg/dL |
Principle
The Beckman Array Protein System in conjunction with the Apolipoprotein (APO) Reagent Test Kit, is intended for in vitro diagnostic quantitation of apolipoprotein in biological fluids by rate nephelometry.
Apolipoproteins (apoproteins) are the protein components of plasma lipoproteins. They compose a heterogeneous group of about 13 major human plasma apoproteins that have been isolated & characterized from chylomicrons, very low density lipoproteins, low density lipoproteins, & high density lipoproteins. Interest in apolipoproteins stems from the greater appreciation of the structure-function relationships of these peptides to the understanding of the special chemistry mechanisms of lipoprotein metabolism & to their possible relationship to disease states. It is now recognized that apolipoproteins are: 1) necessary for the synthesis & catabolism of lipoproteins; 2) necessary structural components of lipoprotein particles for fat transport;
- cofactors or activators of certain enzymes associated with lipid & lipoprotein metabolism; & 4) involved as transfer particles.
Several studies have substantiated an association between lipoprotein fractions & coronary artery disease. This relationship has been helpful in evaluating the pathophysiologic mechanisms involved in atherosclerosis. In addition, specific lipoproteins may be helpful as markers for an increased incidence of coronary artery disease.
Specimen
No special patient preparation is required. 200 uL of serum. Store sample in freezer until ready for assay. Highly lipemic samples may result in inaccurate determination & should be redrawn on a fasting patient.
More general terms
Additional terms
Components
References
- ↑ Beckman Array Protein System Operating Manual.
- ↑ Tietz, Norbert W.: Textbook of Clinical Chemistry, 1986; p. 895-897.
- ↑ Maciejko, Dr. James J.: Apolipoprotein A-I & B & Their Relationship to Coronary Heart Disease.
- ↑ Mayo Clinic Proceedings: Apolipoproteins & Coronary Artery Disease, Vol. 61, May, 1986.
- ↑ 5.0 5.1 Naito, Herbert K.: The Clinical Significance of Apolipoprotein Measurements, Clinical Ligand Assay Society, Inc., Vol. 9, No. 1, Spring 1986.
- ↑ McQueen MJ et al. for the INTERHEART Study Investigators. Lipids, lipoproteins, and apolipoproteins as risk markers of myocardial infarction in 52 countries (the INTERHEART study): A case-control study. Lancet 2008 Jul 19; 372:224 PMID: https://www.ncbi.nlm.nih.gov/pubmed/18640459
- ↑ Panel of 3 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0050028.jsp
Patient information
apolipoprotein A1/B [nephelometry] in serum patient information