protein in body fluid
Principle
The Kodak Ektachem Clinical Chemistry Slide (TP) contains a dry, multilayered analytical element coated on a clear polyester support. The method of analysis is based on the well-known biuret reaction, characterized by the generation of a violet-colored complex when protein is treated with cupric ion (Cu2+) in an alkaline medium. The density of the resulting complex is related to the concentration of total protein in the sample & can be measured spectrophotometrically.
In the Ektachem slide for total protein, the replacement of sodium hydroxide with lithium hydroxide, in combination with a specific copolymer, maintains a high alkalinity & provides excellent keeping stability for the analytical element.
A 10 uL drop of patient specimen is deposited on the slide & is evenly distributed by the spreading layer into the underlying reagent layer.
When the fluid penetrates the layer, diffusion of reagent occurs and the reaction begins. The reaction between protein & copper tartrate takes place largely in the spreading layer where the protein is confined because of its high molecular weight.
The amount of colored complex formed is proportional to the amount of total protein in the sample & is measured by reflected light at 540 nm. Results are reported in grams per deciliter
LiOH Protein + Copper Tartrate -------------> Colored Complex
Specimen
No special patient preparation is required.
For serum preparation: collect whole blood & allow to clot according to manufacturer's instructions. Specimens are collected in a red top vacutainer by venipuncture & should be separated from the cells immediately after collection.
Heparin may be used as an anticoagulant for plasma specimens. Refrigerate specimen if analysis cannot be performed immediately, freeze for long-term storage or shipment.
Plasma Analysis: Results from plasma samples will be up to 0.4 g/dL (4 g/L) higher than serum due to the fibrinogen remaining in the plasma.
Minimum sample size 0.5 milliliter: with an optimum size of 1.0 milliliters or larger.
Interferences
- Preliminary data indicated that hemolysis, icterus and lipemia does interfere with this method.
- Fibrin in the sample may cause metering problems
More general terms
More specific terms
- cryoproteins in serum/plasma
- globulin in body fluid
- protein (total) in serum
- protein in amniotic fluid
- protein in cerebrospinal fluid (CSF)
- protein in dialysis fluid
- protein in gastric fluid
- protein in lower respiratory specimen
- protein in pericardial fluid
- protein in peritoneal fluid; protein in ascites fluid
- protein in pleural fluid
- protein in saliva
- protein in semen
- protein in stool
- protein in synovial fluid
- protein in urine
Component of
- beta-2 globulin/total protein in body fluid
- beta-1 globulin/total protein in body fluid
- beta globulin/total protein in body fluid
- albumin/protein total in body fluid
References
- ↑ Kodak Ektachem 700 Analyzer Operator's Manual, Kodak Clinical Products, Rochester, New York.
- ↑ Kodak Ektachem Slide Package Inserts, Kodak Clinical Products Rochester, New York.
- ↑ Kodak Ektachem Training Manual, Kodak Clinical Products, Rochester, New York.
- ↑ Young, D. S., Pestaner, L. C., Gibberman, V: Effects of drugs on clinical chemistry tests. Clinical Chemistry (Special Issue) 21, April 1975.
- ↑ Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996, pg 11.
- ↑ Total Protein, Body Fluid Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0020502.jsp