lipase in body fluid
Principle
The Kodak Ektachem Clinical Chemistry Slide (LIPA) is a dry, multilayered analytical element coated on a clear polyester support. The analysis is based on an enzymatic method described by Mauck.
A 10 microliter drop of sample is deposited on the slide & is evenly distributed by the spreading layer. The method incorporates colipase which facilitates the absorption of lipase to the substrate micelles in the presence of bile salts. Lipase then catalyzes the hydrolysis of water-insoluble triacetylglycerol esters. The method uses an enzyme diacetinase to convert the substrate 2,3-diacetylglycerol to glycerol. Glycerol kinase converts the glycerol to L-a-glycerolphosphate. L-a -glycerolphosphate oxidase catalyzes the oxidation of l-a -glycerolphosphate to generate hydrogen peroxide. Peroxidase oxidizes a leuco dye to produce a colored dye. The resulting change in reflection density is measured at two fixed times (3.85 and 5.0 minutes). The rate of change in reflection density is proportional to the activity of lipase present in the sample.
Glycerol does not interfere in this method. The two rate measurement times were chosen to ensure that any endogenous glycerol would have reacted before the first read time. However, if the endogenous glycerol concentration is very high, the result will be flagged with a 'DP' error code. In such an event, the sample can be diluted & reanalyzed.
Specimen
Patient Preparation: No special patient preparation is necessary. Sample Preparation: Heparin may be used as an anticoagulant for plasma specimens. EDTA, oxalate/fluoride & citrate should not be used as anticoagulants. Collect the specimens by standard venipuncture technique. Remove serum promptly from the clot & analyze as soon as possible. Ensure equipment is free from soap or glycerol contamination. Collection tubes with glycerol lubricated stoppers should not be used. Refrigerate specimens if it cannot be analyzed immediately. Freeze specimen for long-term storage or shipment.
Sample requirements:
More general terms
More specific terms
- lipase in amniotic fluid
- lipase in dialysis fluid
- lipase in peritoneal dialysis fluid
- lipase in peritoneal fluid
- lipase in pleural fluid
- lipase in serum
Additional terms
References
- ↑ Kodak Ektachem 700 Test Methodologies Manual, Kodak Clinical Products, Rochester, N.Y., 1990.
- ↑ Kodak Ektachem 700 Operators Manual, Kodak Clinical Products, Rochester, N.Y., 1987.
- ↑ Package Insert, Kodak Ektachem Special Calibrators, Kodak Clinical Products, Rochester, N.Y., 1985.
- ↑ Package Insert, Bio-Rad Liquichek Controls, Bio-Rad ECS Division, Anaheim, CA., 1992.
- ↑ Medical Knowledge Self Assessment Program (MKSAP) 11, 15, American College of Physicians, Philadelphia 1998, 2009
- ↑ Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996, pg 11.
- ↑ Lipase, Fluid Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0020715.jsp