Splicing factor U2AF 26 kD subunit (U2 small nuclear RNA auxiliary factor 1-like protein 4, U2 small nuclear RNA auxiliary factor 1-like protein 3, U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3, U2AF1-like protein 3, U2AF1L4, U2AF-RS3, U2AF1L3)
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Function
- RNA-binding protein
- functions as a pre-mRNA splicing factor
- role in both constitutive & enhancer- dependent splicing by mediating protein-protein interactions & protein-RNA interactions required for accurate 3'-splice site selection
- acts by enhancing the binding of U2AF2 to weak pyrimidine tracts
- role in regulation of alternative pre-mRNA <A14277>splicing</A14277>
- activates exon 5 skipping of PTPRC during T cell activation; an event reversed by GFI1
- binds to RNA at the AG dinucleotide at the 3'-splice site (putative)
- interacts with GFI1 & U2AF2 (putative)
Structure
- belongs to the splicing factor SR family
- contains 2 C3H1-type zinc fingers, 2nd zinc finger in necessary for interaction with GFI1 & for alternative pre-mRNA splicing events (putative)
- contains 1 RRM (RNA recognition motif) domain
Compartment
nucleus (putative), nuclear speckle
Alternative splicing
named isoforms=3
Expression
- isoform 2 is widely expressed
- isoform 3 is expressed in heart, brain & lung > thymus > peripheral blood leukocytes