matrix metalloproteinase-3 (matrixin-3, stromelysin-1, transin-1, SL-1, MMP3, STMY1)

Function

• degradation of
  • fibronectin
  • laminin
  • gelatins of type 1, 3, 4, & 5
  • collagens 3, 4, 9, 10
  • cartilage proteoglycans
• activates procollagenase
• preferential cleavage where P1', P2' & P3' are hydrophobic residues

Cofactor:

• binds 4 Ca+2 per subunit
• binds 2 Zn+2 per subunit

Structure

• conserved Cys present in the cysteine-switch motif binds the catalytic Zn+2, thus inhibiting the enzyme; dissociation of the Cys from the Zn+2 upon the activation-peptide release activates the enzyme
• belongs to the peptidase M10A family
• contains 4 hemopexin-like domains

Laboratory

• stromelysin-1 in serum/plasma

More general terms

• matrixin or matrix metalloproteinase
• glycoprotein
• Ca+2 binding protein

Database

• UniProt: http://www.uniprot.org/uniprot/P08254.html
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:4314
• OMIM: https://mirror.omim.org/entry/185250
• OMIM: https://mirror.omim.org/entry/164015
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=4314