chloride intracellular channel protein 5 (CLIC5)

Function

• can insert into membranes & form poorly selective ion channels that may also transport chloride ions
• may play role in regulation of transepithelial ion absorption & secretion
• required for normal formation of stereocilia in the inner ear & normal development of the organ of corti (putative)
• required for the development &/or maintenance of proper glomerular endothelial cell & podocyte architecture
• component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, & IQGAP1
• interacts with AKAP9

Structure

• members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane & functions as chloride channel
• a conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (putative)
• belongs to the chloride channel CLIC family
• contains 1 GST C-terminal domain

Compartment

• isoform 1:
  • cytoplasm, cell cortex, cytoskeleton, membrane
  • single-pass membrane protein
  • associates with the cortical actin cytoskeleton
  • exists both as soluble cytoplasmic protein & as membrane protein
• isoform 2
  • Golgi, cytoplasm, cytoskeleton, centrosome, membrane
  • single-pass membrane protein
  • colocalized with AKAP9 at the Golgi & the centrosome (in part)
  • exists both as soluble cytoplasmic protein & as membrane protein
  • colocalized with podocalyxin in renal glomeruli

Alternative splicing

named isoforms=2

Expression

• isoform 1: expressed in renal glomeruli endothelial cells & podocytes (at protein level)

More general terms

• chloride intracellular channel protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=53405
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:53405
• OMIM: https://mirror.omim.org/entry/607293
• UniProt: http://www.uniprot.org/uniprot/Q9NZA1.html