carbohydrate sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; keratan sulfate gal-6 sulfotransferase; KS6ST; KSgal6ST; KSST (CHST1)

Function

• catalyzes transfer of sulfate to position 6 of galactose residues of keratan
• preference for sulfating keratan sulfate, but also transfers sulfate to the unsulfated polymer
• sulfotransferase activity on sialyl lacNAc structures is much higher than the corresponding desialylated substrate, & only internal galactose residues are sulfated
• may function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins
• participates in biosynthesis of selectin ligands
• selectin ligands are present in high endothelial cells & play a central role in lymphocyte homing at sites of inflammation

3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate

Structure

• belongs to the sulfotransferase 1 family gal/GlcNAc/galNAc subfamily

Compartment

• Golgi membrane
• single-pass type 2 membrane protein (putative)

Expression

• widely expressed at low level
• expressed in brain & skeletal muscle
• expressed by high endothelial cells & leukocytes

More general terms

• carbohydrate sulfotransferase (CHST)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=8534
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:8534
• OMIM: https://mirror.omim.org/entry/603797
• UniProt: http://www.uniprot.org/uniprot/O43916.html