E3 ubiquitin-protein ligase Praja-1; Praja1; RING finger protein 70 (PJA1, RNF70)

Function

• E2-dependent E3 ubiquitin-protein ligase
• ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteosome (putative)
• may be involved in protein sorting
• substrate for E2-dependent ubiquitination
• binds ubiquitin-conjugating enzymes (E2s)
• in vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2

Structure

• the RING-type Zn+2 finger domain interacts with an ubiquitin-conjugating enzyme (E2) & facilitates ubiquitination
• contains 1 RING-type Zn+2 finger

Alternative splicing

• named isoforms=2
• isoform 2 may arise by alternative initiation

Expression

• expressed in various regions of the brain, including cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe & putamen
• highest levels in the cerebral cortex

More general terms

• E3 ubiquitin ligase
• zinc finger protein
• phosphoprotein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=64219
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:64219
• OMIM: https://mirror.omim.org/entry/300420
• UniProt: http://www.uniprot.org/uniprot/Q8NG27.html