tripartite motif-containing protein 25; zinc finger protein 147; estrogen-responsive finger protein; Efp; RING finger protein 147 (TRIM25, EFP, RNF147, ZNF147)

Function

• ubiquitin E3 ligase & ISG15 E3 ligase
• role in innate immune defense against viruses by mediating ubiquitination of DDX58
• mediates Lys-63-linked polyubiquitination of the DDX58 N-terminal CARD-like region
  • this triggers cytosolic signal transduction leading to production of interferons in response to viral infection
• promotes ISGylation of 14-3-3 sigma
• mediates estrogen action in various target organs
• protein modification; protein ubiquitination
• auto-ISGylated
• interacts (via SPRY domain) with DDX58 (via CARD domain)
• interacts (via coiled coil) with influenza A virus NS1 protein
  • this interaction specifically inhibits TRIM25 multimerization & RIM25-mediated DDX58 CARD ubiquitination, thus suppressing DDX58 signal transduction

Structure

• the RING-type Zn+2 finger is important for ISG15 E3 ligase activity & auto-ISGylation
  • auto-ISGylation negatively regulates ISG15 E3 ligase activity
• the C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58
• contains 1 B30.2/SPRY domain
• contains 1 RING-type Zn+2 finger

Compartment

• cytoplasm
• colocalized with DDX58 at cytoplasmic perinuclear bodies

Expression

• ubiquitous
• induced by interferons

More general terms

• ring finger protein
• phosphoprotein
• human longevity protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=7706
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:7706
• OMIM: https://mirror.omim.org/entry/600453
• UniProt: http://www.uniprot.org/uniprot/Q14258.html