carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1; nuclear LIM interactor-interacting factor 3; NLI-IF; NLI-interacting factor 3; small C-terminal domain phosphatase 1; SCP1; small CTD phosphatase 1 (CTDSP1, NIF3, NLIIF, SCP1)

Function

• preferentially catalyzes dephosphorylation of Ser-5 within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase 2 subunit POLR2A
• negatively regulates RNA polymerase 2 transcription, possibly by controlling transition from initiation/capping to processive transcript elongation
• recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells
• stimulated by GTF2F1
• inhibited by beryllofluoride anions
• interacts with GTF2F1
• interacts with REST

pH dependence: optimum pH is 5

Cofactor: binds 1 Mg+2 per monomer

Structure

• monomer (putative).
• contains 1 FCP1 homology domain

Compartment

• nucleus
• colocalizes with RNA polymerase 2

Alternative splicing

named isoforms=2

Expression

• expression is restricted to non-neuronal tissues
• highest expression in skeletal muscle, spleen, lung & placenta

More general terms

• NLI-interacting factor

Additional terms

• LIM domain
• LIM domain protein kinase

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=58190
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:58190
• OMIM: https://mirror.omim.org/entry/605323
• UniProt: http://www.uniprot.org/uniprot/Q9GZU7.html