FACT complex subunit SSRP1; facilitates chromatin transcription complex subunit SSRP1; FACT 80 kD subunit; FACTp80; chromatin-specific transcription elongation factor 80 kD subunit; structure-specific recognition protein 1; hSSRP1; recombination signal sequence recognition protein 1; T160 (SSRP1, FACT80)

Function

• component of the FACT complex
• also acts as a transcriptional coactivator for p63/TP63
• phosphorylated by CK2 following UV but not gamma irradiation; phosphorylation inhibits its DNA-binding activity
• ubiquitinated; polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein
• sumoylated
• also component of a CK2-SPT16-SSRP1 complex
• binds to histone H3-H4 tetramers, but not to intact nucleosomes
• interacts with isoform gamma of p63/TP63
• interacts with SRF & NEK9

Structure

• the HMG box DNA-binding domain mediates DNA-binding
• has both affinity & specificity for DNA damaged globally with cisplatin
• belongs to the SSRP1 family
• contains 1 HMG box DNA-binding domain

Compartment

• nucleus
• colocalizes with RNA polymerase 2 on chromatin
• recruited to actively transcribed loci

Pathology

• autoantibodies against SSRP1 are present in sera from patients with systemic lupus erythematosus, but not other rheumatic diseases

More general terms

• DNA-binding protein
• phosphoprotein

Component of

• FACT complex
• CK2-SPT16-SSRP1 complex

Database

• UniProt: http://www.uniprot.org/uniprot/Q08945.html
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=6749
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:6749
• OMIM: https://mirror.omim.org/entry/604328