pyruvate kinase M-type (PKM)

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Function

ATP + pyruvate <--> ADP + phosphoenolpyruvate

Cofactors: Mg+2, K+

Kinetic parameters:

  • KM=2.7 mM for phosphoenolpyruvate#
  • KM=0.17 mM for phosphoenolpyruvate (in the presence of 2mM FBP)
  • KM=0.34 mM for ADP
  • KM=0.24 mM for ADP (in the presence of 2mM FBP)

# at pH=8 & 32 degrees celsius

inhibited by oxalate

Structure

homotetramer belongs to the pyruvate kinase family

Alternative splicing

named isoforms=2

Expression

  • early fetal tissues as well as in most cancer cells

Genetics

Notes

  • 4 isozymes of pyruvate kinase in mammals: L, R, M1 & M2
  • L type is major isozyme in the liver
  • R is found in red cells
  • M1 is the main form in muscle, heart & brain
  • M2 is found in early fetal tissues as well as in most cancer cells

More general terms

More specific terms

References

  1. UniProt http://www.uniprot.org/uniprot/P14786.html
  2. Wikipedia; Note: pyruvate kinase entry http://en.wikipedia.org/wiki/pyruvate_kinase
  3. Stryer Biochemistry WH Freeman & Co, New York,1988 pg 362
  4. Wikipedia; Note: pyruvate kinase entry http://en.wikipedia.org/wiki/pyruvate_kinase

Database