SH3 domain-binding protein 4 (transferrin receptor-trafficking protein, EH-binding protein 10, SH3BP4, BOG25, EHB10, TTP)

Function

• role in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin- mediated endocytosis
• interacts with DNM2, EPS15, clathrin, adapter protein complex 2/AP-2 & TFRC
• phosphorylated upon EGF stimulation phosphorylation prevents interaction with DNM2
• overexpression or depletion of SH3BP4 result in a specific decrease of the transferrin receptor endocytosis that can be rescued by DNM2 overexpression

Structure

• homodimer or homooligomer
• contains 1 SH3 domain that mediates
  • localization to clathrin-coated pits & vesicles
  • interaction with DNM2 & cytoplasmic part of TFRC

Compartment

• cell membrane, clathrin-coated pit
• cytoplasmic vesicle, clathrin-coated vesicle
• specifically associated with transferrin receptor- containing clathrin-coated pits & clathrin-coated vesicles
• may also localize to nucleus

Alternative splicing

named isoforms=2

Expression

• expressed in all tissues tested
• higher expression in pancreas
• expressed by retinal pigment epithelial cells (at protein level)

More general terms

• membrane protein
• phosphoprotein
• SH3 domain-binding protein

Database

• OMIM: https://mirror.omim.org/entry/605611
• UniProt: http://www.uniprot.org/uniprot/Q9P0V3.html