meprin A subunit alpha; endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha (MEP1A)

Function

• hydrolysis of protein & peptide substrates preferentially on carboxyl side of hydrophobic residues
• homotetramer consisting of disulfide-linked alpha subunits
• homooligomer consisting of disulfide-linked alpha subunit homodimers
• heterotetramer of two alpha & two beta subunits formed by non-covalent association of two disulfide-linked heterodimers (putative)
• interacts with MBL2 through its carbohydrate moiety; interaction may inhibit its catalytic activity (putative)

Inhibition:

• inhibited by several hydroxamate compounds, the most potent inhibitor is actinonin

Kinetic parameters:

• KM=110 uM for GRP
• KM=18.0 uM for PTH 12-34
• KM=33.9 uM for secretin
• KM=41.3 uM for substance P
• KM=56.5 uM for LHRH
• KM=73.2 uM for orcokinin
• KM=292 uM for alpha-MSH
• KM=125 uM for bradykinin
• KM=200 uM for gastrin

Cofactor:

• binds 1 Zn+2 per subunit (putative)

Structure

• N-glycosylated; contains GlcNAc, galactose, mannose & a small amount of fucose (putative)
• belongs to the peptidase M12A family
• contains 1 EGF-like domain
• contains 1 MAM domain
• contains 1 MATH domain

Compartment

• membrane; single-pass type 1 membrane protein

More general terms

• plasma membrane protein
• glycoprotein
• protein subunit

Component of

• meprin (endopeptidase-2)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=4224
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:4224
• OMIM: https://mirror.omim.org/entry/600388
• UniProt: http://www.uniprot.org/uniprot/Q16819.html