lipoyl synthase, mitochondrial; lipoate synthase; LS; lip-syn; lipoic acid synthase (LIAS, LAS, HUSSY-01)

Function

• catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 & C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thus converting the octanoylated domains into lipoylated derivatives
• protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2

protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine

Cofactor:

• binds 2 4Fe-4S clusters per subunit
• one cluster is coordinated with 3 Cys & an exchangeable S-adenosyl-L-methionine (putative)

Structure

• belongs to the radical SAM superfamily, lipoylsynthase family

Compartment

mitochondria (putative)

More general terms

• sulfurtransferase
• mitochondrial protein
• metalloprotein

Additional terms

• HUSSY protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=11019
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:11019
• OMIM: https://mirror.omim.org/entry/607031
• UniProt: http://www.uniprot.org/uniprot/O43766.html