bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; includes: NAD-dependent methylenetetrahydrofolate dehydrogenase; methenyltetrahydrofolate cyclohydrolase (MTHFD2, NMDMC)

Function

• bifunctional enzyme

5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH

5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate

Cofactor: Mg+2

Structure

• homodimer
• belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Compartment

mitochondria

Expression

• expressed only in developing normal tissues

Notes

• has very different kinetic properties than the larger NADP-dependent trifunctional enzyme & is unique in that it requires formation of an enzyme-Mg+2 complex to allow binding of NAD

More general terms

• dehydrogenase
• mitochondrial protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=10797
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:10797
• OMIM: https://mirror.omim.org/entry/604887
• UniProt: http://www.uniprot.org/uniprot/P13995.html