lariat debranching enzyme (DBR1)

Function

• RNA processing activity that hydrolyzes the 2'-5' phosphodiester linkage at the branchpoint of excised intron lariats (after splicing)
  • converts them into linear molecules that are subsequently degraded
• facilitates ribonucleotide turnover

Cofactor: divalent metal cations

Kinetic parameters: Optimum pH is7.0

Compartment

nucleus

Alternative splicing

named isoforms=2

Pathology

• retrovirus replication via a RNA lariat intermediate in cDNA synthesis
• cells lacking DBR1 show an inhibition of HIV-1 replication

More general terms

• hydrolase
• nuclear protein

Database

• OMIM: https://mirror.omim.org/entry/607024
• UniProt: http://www.uniprot.org/uniprot/Q9UK59.html