FACT complex subunit SPT16 (facilitates chromatin transcription complex subunit SPT16, hSPT16, FACT 140 kD subunit, FACTp140, chromatin-specific transcription elongation factor 140 kD subunit, SUPT16H, FACT140, FACTP140)

Function

• component of FACT complex
• component of a CK2-SPT16-SSRP1 complex
• interacts with NEK9
• binds to histone H2A-H2B
• interacts with GTF2E2
• ADP-ribosylation by PARP1 is induced by genotoxic stress & correlates with dissociation of FACT from chromatin

Structure

• Glu-rich acidic region in C-terminus is essential for FACT complex activit
• ADP-ribosylated
• belongs to the peptidase M24 family. SPT16 subfamily although related to the peptidase M24 family, lacks conserved active site residues, thus lacks peptidase activity

Compartment

• nucleus
• colocalizes with RNA polymerase 2 on chromatin.
• recruited to actively transcribed loc

Expression

• ubiquitous

More general terms

• protein subunit

Database

• OMIM: https://mirror.omim.org/entry/605012
• UniProt: http://www.uniprot.org/uniprot/Q9Y5B9.html