protein kinase C-beta (PKC-II, PRKCB1, PKCB, PRKCB)

Function

• Ca+2-activated, phospholipid-dependent
• serine/threonine kinase
• activated by diacylglycerol
• phosphorylates a range of cellular proteins
• component of the NF-kappa-B signaling axis responsible for survival & activation of B-cells after BCR cross-linking
• interacts with PDK1, PRKCBP1
• phosphorylation on Thr-500 of isoform beta-1, within the activation loop, renders it competent to autophosphorylate
  • subsequent autophosphorylation of Thr-642 maintains catalytic competence, & autophosphorylation on Ser-661 appears to release the kinase into the cytosol
• isoform beta-2 is autophosphorylated on Thr-640 & Ser-659, subsequent to phosphorylation on Thr-500
• autophosphorylated on other sites i.e in the N-terminal & hinge regions have no effect on PKC activity

Cofactor:

• binds 3 Ca+2 per subunit
• Ca+2 bound to the C2 domain

Structure

• belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
• contains 1 AGC-kinase C-terminal domain
• contains 1 C2 domain
• contains 2 phorbol-ester/DAG-type Zn+2 fingers
• contains 1 protein kinase domain

Compartment

cytoplasm, peripheral membrane

Alternative splicing

named isoforms=2: PRKCB1, PRKCB2

More general terms

• protein kinase C type-A
• zinc finger protein

More specific terms

• protein kinase C-beta 1 (PKC-IIa, PRKCB1)
• protein kinase C-beta 2 (PKC-IIb)

Database

• UniProt: http://www.uniprot.org/uniprot/P05771.html
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:5579
• OMIM: https://mirror.omim.org/entry/176970
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=5579