replication initiation-like protein (myelin transcription factor 2, MyT2, cerebrin-50, MYT2)
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Function
- may be involved in DNA replication
- binds to the promoter of the myelin proteolipid protein PLP1 gene
- preferentially binds supercoiled DNA
- reduces the number of supercoils in a highly negatively supercoiled DNA via a non-enzymatic mechanism
- binds also to methylated & non-methylated single-stranded oligonucleotides corresponding to the Sp1 binding site of HIV-1 LTR
Structure
- highly similar to microbial proteins
Compartment
- nucleus, cytoplasm, secreted
- detected in cerebrospinal fluid & in T-lymphoblastoid cell culture medium & serum
Alternative splicing
named isoforms=2
Expression
- expressed in brain
- expressed in oligodendrocyte progenitor cells, pyramidal cells & purkinje neurons, neurons of the hypothalamus, hippocampus, ventromedial thalamus, cells of the choroid plexus & ciliated ependymal cells (at protein level)
- expressed predominantly in brain & a T-lymphoblastoid cell line, moderately in spleen, liver & testis
- mRNA transcript encoding MYT2 contains an internal ribosome entry site (IRES) in order to promote its translation
- belongs to the low-abundance category of cellular transcripts undetectable by Northern blot or Rnase protection assays
Notes
- MYT2 has not yet been found to be encoded in the human genome