E3 ubiquitin-protein ligase Arkadia (RING finger protein 111, RNF111)

Function

• role in the NODAL pathway of mesoderm patterning during embryonic development
• acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs including SMAD7, induces their proteasomal degradation & thus enhances the transcriptional activity of TGF-beta & BMP.
• ubiquitin conjugation, 3rd step
• interacts with SMAD6, SMAD7, AXIN1 & AXIN2
• component of a complex containing RNF111, AXIN1 & SMAD7

Structure

contains 1 RING-type zinc finger

Compartment

• nucleus, cytoplasm
• upon TGF-beta treatment, translocates from nucleus to cytosol

Alternative splicing

named isoforms=3

Expression

broadly expressed

More general terms

• E3 ubiquitin ligase
• ring finger protein

Database

• OMIM: https://mirror.omim.org/entry/605840
• UniProt: http://www.uniprot.org/uniprot/Q6ZNA4.html