peroxisomal NADH pyrophosphatase NUDT12; nucleoside diphosphate-linked moiety X motif 12; Nudix motif 12 (NUDT12)

Function

• hydrolyzes NADH to nicotinamide & AMP
• hydrolyzes NADPH to nicotinamide & 2',5'-ADP
• hydrolyzes ADP to AMP
• has activity towards NAD+, NADP+, ADP-ribose & ATP
• may act to regulate peroxisomal NAD+, NADP

Cofactor: Mg+2 or Mn+2

Kinetic parameters:

• KM=11 uM for NADH
• KM=16 uM for NADPH
• KM=190 uM for NAD
• optimum pH is 8-9

Structure

• belongs to the Nudix hydrolase family, NudC subfamily
• contains 3 ANK repeats
• contains 1 nudix hydrolase domain

Compartment

peroxisome

More general terms

• pyrophosphatase (diphosphohydrolase)
• nucleoside diphosphate-linked moiety X motif

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=83594
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:83594
• OMIM: https://mirror.omim.org/entry/609232
• UniProt: http://www.uniprot.org/uniprot/Q9BQG2.html