D-3-phosphoglycerate dehydrogenase; 3-PGDH (PHGDH, PGDH3)

Function

• amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3

3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH

2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH

Kinetic parameters:

• KM=21.6 uM for 3-phosphohydroxypyruvate
• Vmax=35-168 nmol/min/mg enzyme with 3-phosphohydroxypyruvate as substrate

Structure

• homotetramer (putative)
• belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Expression

• induced by 17-beta-estradiol (estrogenic ligand) & 4-hydroxytamoxifen (agonist/antagonist ligand)
• positively regulated by the transcription factors SP1 & NF-Y

Pathology

• defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency

More general terms

• dehydrogenase
• phosphoprotein

Additional terms

• 3-phosphoglycerate
• phosphoglycerate dehydrogenase deficiency

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=26227
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:26227
• OMIM: https://mirror.omim.org/entry/601815
• OMIM: https://mirror.omim.org/entry/606879
• UniProt: http://www.uniprot.org/uniprot/O43175.html