cytosolic beta-glucosidase; cytosolic beta-glucosidase-like protein 1 (GBA3, CBG, CBGL1)

Function

• glycosidase
• role in intestinal absorption & metabolism of dietary flavonoid glycosides
• hydrolyzes variety of glycosides including phytoestrogens, flavonols, flavones, flavanones & cyanogens
• beta-glycosylceramidase activity
  • may be involved in a nonlysosomal catabolic pathway of glycosylceramide
• hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose

Inhibition:

• inhibited by
  • 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside
  • sodium taurocholate

Kinetic parameters:

• KM=10 uM for luteolin-4'-glucoside;
• KM=50 uM for luteolin-7-glucoside;
• Vmax=1.30 umol/min/mg enzyme toward luteolin-4'-glucoside;
• Vmax=2.85 umol/min/mg enzyme toward luteolin-7-glucoside;
• Optimum pH is 6.5, active from pH 5 to 7.5

Structure

• belongs to the glycosyl hydrolase 1 family, Klotho subfamily
• N-terminus is blocked

Compartment

cytoplasm, cytosol

Alternative splicing

named isoforms=2

Expression

• expressed in liver, small intestine, colon, spleen & kidney

Pathology

• down-regulated in
  • renal cell carcinomas
  • hepatocellular carcinomas

More general terms

• glucosidase
• human longevity protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=57733
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:57733
• OMIM: https://mirror.omim.org/entry/606619
• UniProt: http://www.uniprot.org/uniprot/Q9H227.html